Part:BBa_K4612120
LY6E_S
LY6E _S (BBa_K4612120) is a 107-nucleotide single-stranded DNA sequence that is a weighted probe for the target LY6E. The 3' end of the sequence is designed to be complementary to the target LY6E so that it binds specifically to the target and thus recognizes the input signal.DNA polymerase polymerizes the DNA in the 5'-3' direction starting from the 3' end of the target LY6E.The probe also binds to a probe corresponding to the target LY6E. The probe also incorporates a weighted strand corresponding to weight size 4 of the target LY6E, which is replaced and released by the newly synthesized daughter strand during DNA polymerization by Bst DNA polymerase. Through this process, the signal of the target is quantitatively converted into the number of weighted strands, which facilitates subsequent calculations, and finally the results are converted into different fluorescence intensities, making the results easy to read.
Sequence and Features
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